Determination of nifedipine in serum or plasma by reversed-phase liquid chromatography.
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چکیده
منابع مشابه
Simultaneous determination of retinol and retinyl esters in serum or plasma by reversed-phase high-performance liquid chromatography.
We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for r...
متن کاملDetermination of monosaccharides in cider by reversed-phase liquid chromatography
A reversed-phase high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of aldoses and uronic acids in cider, previously derivatized with p-aminobenzoic ethyl ester (ABEE). Narrow-bore C8 columns are recommended as this alternative provides good separation efficiency, along with greater economy and sensitivity. Detection limits for aldoses (glucose...
متن کاملDetermination of Trazodone in Human Plasma by Reversed-phase Liquid Chromatography-mass Spectrometry with Electrospray Ionisation
Objective: The development and validation of LC-MS/MS method for quantification of Trazodone (a serotonin antagonist and reuptake inhibitor (SARI), which is a second generation antidepressant compound belonging to the class of phenyl piperazine) in human plasma is described. Methods: The method involves protein precipitation (extraction) using Trazodone d6 as an internal standard (IS). Chromato...
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In this novel method for quantifying bethanidine in plasma, after a multi-step extraction of bethanidine and internal standard from 2.0 mL of plasma, the drugs are separated on a "microbore" C18 reversed-phase column and quantified by their ultraviolet absorbance at 210 nm. The isocratic chromatographic separation takes about 15 min with use of an ion-pairing regent in the mobile phase (acetate...
متن کاملDetermination of clonixin in plasma and urine by reversed-phase high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method that enables the determination of clonixin in human plasma and urine samples is described. Recovery of the drug was over 87.6 and 80.7% for plasma and urine, respectively. The limit of quantitation of the method was established as 10 ng/ml in plasma and 20 ng/ml in urine samples, with RSDs of less than 11.1%. The applicability of t...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1983
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/29.7.1344